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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Construction of tandem diabody (IL-6/CD20)-secreting human umbilical cord mesenchymal stem cells and its experimental treatment on diffuse large B cell lymphoma

Fig. 3

The expression of Tandab protein. (A) The relative expression of Tandab mRNA in UCMSCs-Tandab(IL-6/CD20), UCMSCs-Vector and UCMSCs analyzed by qRT-PCR. (B-C) The relative expression of Tandab protein in the cell lysis (B) and the culture supernatant (C) of UCMSCs-Tandab(IL-6/CD20) by western blot. (D) Representative immunofluorescence staining of UCMSCs-Tandab(IL-6/CD20), UCMSCs-Vector and UCMSCs. Green and red represent spontaneous fluorescence and 6 × His Tag, respectively. (E–F) After collecting the culture supernatants of UCMSCs-Tandab(IL-6/CD20), the Tandab protein level was measured by ELISA. n = 3 for each group. ns: not significant, ***p < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512