Fig. 3

Extracellular matrix protein and enzyme expression in equine MSCs. a Heatmap representation of extracellular matrix protein genes from RNA-sequencing for untreated and TGF-β-treated equine MSCs. Data shown are Z scores, log₂ fold change, and the adjusted p value for each gene for n = 4. b Representative western blot of TNC and ACTB protein expression in untreated and TGF-β2-treated equine MSCs and the densitometric comparison of the relative expression of TNC to ACTB. Data shown are mean ± SD for n = 4, ***p = 0.0005 by a one-tailed t test. c Heatmap representation of extracellular matrix enzyme genes from RNA-sequencing for untreated and TGF-β2-treated equine MSCs. Data shown are Z scores, log₂ fold change, and the adjusted p value for each gene for n = 4