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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: A homozygous PIWIL2 frameshift variant affects the formation and maintenance of human-induced pluripotent stem cell-derived spermatogonial stem cells and causes Sertoli cell-only syndrome

Fig. 3

Transcriptome analysis of cells at 0, 8, and 12 days of SSCLC induction. A Volcano plots of comparison of transcriptome between the control and PIWIL2−/− groups at 8 and 12 days of differentiation. Red dots represented upregulated genes, green dots represented downregulated genes, and black dots represented genes not significantly changed. B KEGG analysis on the downregulated genes at 8 and 12 days of differentiation. C The expression of genes in Wnt signaling pathway, pluripotency, SSC, spermatocyte, endoderm, mesoderm, and ectoderm in the control and PIWIL2−/− groups at 0, 8, and 12 days of differentiation. D RT-qPCR confirmed the expression of genes in Wnt signaling pathway and pluripotency in the control and PIWIL2−/− groups at 8 and 12 days of differentiation. n = 3, * versus Control, # versus PIWIL2+/−, p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512