Fig. 1
The characterization of MSC MV. MSCs were treated by serum starvation for 48 h. A Scanning electron microscopy showed that there were microvesicles sprouting and falling off, and the shape was like a round, vesicle-like around the surface of the starved MSCs. B Microvesicles were collected by ultracentrifugation. Scanning electron microscope showed that microvesicles were in the form of vesicles and had a double-layer membrane structure. C NTA analysis of the obtained microvesicles found that 99.2% of the microvesicles were about 136.9 nm in size, and the concentration was about 1.9 × 1011 per ml. D Western blot analysis on the expression of the MV markers CD63 and TSG101. All experiments were repeated three times. MSC, mesenchymal stem cell; MV, microvesicle; NTA, nanoparticles tracking technology