Fig. 6
MSC MV promoted Ca2+ mobilization in injured HLMVEC by increasing its intracellular S1P. A Fluorescence was observed after HLMVEC treated with Ca2+ probe Flu-3AM by laser confocal. B Intracellular calcium fluorescence intensity in different groups was analyzed by ImageJ. Data are presented as mean ± SD, N = 3. *p < 0.05 versus control using ANOVA with post hoc Tukey HSD test. MSC, mesenchymal stem cell; MV, microvesicles; HLMVEC, human lung microvascular endothelial cells; S1P, sphingosine-1-phosphate; and ANOVA, analysis of variance