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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: TNFα and IFNγ rapidly activate PI3K-AKT signaling to drive glycolysis that confers mesenchymal stem cells enhanced anti-inflammatory property

Fig. 5

PI3K-AKT signaling is required for the increase of HKII activity. a The protein level of HKII is not changed upon TNFα and IFNγ stimulation. b HKII activity is increased quickly after TNFα and IFNγ stimulated. c MSCs were pretreated with LY294002 (LY) or triciribine (TCN) for 1 h and then TNFα and IFNγ were added into the medium for 1 h. HKII activity of MSCs was analyzed by hexokinase activity assay. d MSCs were infected by adenovirus expressing constitutive active form Myr-AKT or dominant-negative AKT (DN-AKT) two days before TNFα and IFNγ stimulation. After TNFα and IFNγ treatment for 1 h, HKII activity of MSCs was analyzed by hexokinase activity assay. Data are presented as mean ± SEM. of triplicates (b–d) ***, p < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512