Fig. 4

Comparation of cell vitality and cytokine secretion of hAMSCs under SC and SF conditions. a The proliferation of hAMSCs under SC and SF conditions. b-c Representative FCM diagrams (b) and statistical analysis (c) of Ki-67 MFI (mean fluorescence intensity) in hAMSCs under SC and SF conditions. d-e Representative FCM diagrams (d) and statistical analyses (e) of the distributions of the sub-stages of cell cycle in hAMSCs under SC and SF conditions. f–g Representative FCM diagrams (f) and statistical analyses (g) of the pre-apoptotic or apoptotic property of hAMSCs under SC and SF conditions. h Relative mRNA expression of the indicated cytokines in hAMSCs under SC and SF conditions. i The proliferation of hAMSCs under SC, SF, and SF + Da (10 nM 1,3-dicaffeoylquinic acid for PI3K-AKT-mTOR reactivation, denote as “Da”) conditions. j–k Representative FCM diagrams (j) and statistical analysis (k) of Ki-67 MFI in hAMSCs under SC, SF, and SF + Da conditions. l Representative G-banded chromosome karyotyping of hAMSCs under SC and SF conditions. Data were shown as mean ± SEM (N = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; NS, not significant