Fig. 5

Comparation of the ex vivo immunoregulatory property of hAMSCs under SC and SF conditions. a–b The inhibitory effects of hAMSCs preconditioned in SC and SF conditions for 48 h upon CD3⁺ T cells (a), CD4⁺CD44⁺ T cells (left panel), CD8⁺CD44⁺ T cells (right panel) (b). c–d The representative diagrams (left panel) and statistical analyses (right panel) of CD4⁺CD69⁺ T cells (c) and CD8⁺CD69⁺ T cells (d) cocultured without (T only) or with hAMSCs preconditioned under SC and SF conditions. e–f The proportions of CD45RO⁺ and CD45RA⁺ subsets in CD4⁺ T cells (e) and CD8⁺ T cells (f) in the indicated groups. g–i The proportions of CD4⁺Foxp3⁺ Treg (g), CD8⁺IFN-γ⁺ Tc1 (h), and CD8⁺IL-4⁺ Tc2 (i) subpopulations in the indicated groups. j-l The proportion of CD4⁺IFN-γ⁺ Th1 (j) and CD4⁺IL-4⁺ Th2 (k) and CD4⁺IL-17A⁺ Th17 (l) cells in the indicated groups. m–n Statistical analysis of the cell growth (m) and apoptosis (n) of the cocultured CD3⁺ T lymphocyte subpopulations with hAMSCs preconditioned in SC and SF conditions for 48 h. Data were shown as mean ± SEM (N = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; NS, not significant