Fig. 3

CPP promoted the expression of CD133. A–B HDFs were treated with 0, 1, 10 or 20 μM CPP for 10 days (D10), after which the protein level of CD133 was determined by Western Blot. β-actin (ACTB) was used as a loading control. Quantitation of bands in the Western blots (A) is shown in (B). (C) Different doses of CPP (0, 1, 10 or 20 μM) were used to treat HDFs for 10 days, after which mRNA levels of CD133 were detected by qPCR. (D) Different doses of CPP (0, 1, 10 or 20 μM) were used to treat HDFs for 10 days (D10), after which protein levels of CD133 were detected by immunofluorescence. Scale bar: 20 μm. Data are presented as means ± SEM, *P < 0.05, **P < 0.01, n = 3