Fig. 4

CPP promoted the expression of CD31. A–B HDFs were treated with 0, 1, 10 or 20 μM CPP for 10 days (D10), and the protein level of CD31 was determined by Western Blot. β-actin (ACTB) was used as a loading control. Quantitation of bands in the Western blots (A) is shown in (B). C Different doses of CPP (0, 1, 10 or 20 μM) were used to treat HDFs for 10 days, and the mRNA levels of CD31 were detected by qPCR. D Different doses of CPP (0, 1, 10 or 20 μM) were used to treat HDFs for 10 days (D10), after which protein level of CD31 was detected by immunofluorescence. Scale bar: 20 μm. Data are presented as means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3