Fig. 7

VECs derived from HDFs have the function of angiogenesis in vivo. A HDFs were treated with 0.1% DMSO (as a control) or with CPP for 10 days, and then treated cells and HUVECs were labeled by CM-DiI. Labeled cells were seeded into CAM. One week later, the fluorescence was detected by the laser scanning confocal microscope Zeiss LSM900 (Germany). Scale bar: 50 μm. B The number of cells incorporated into vessels was counted by ImageJ. One million CPP-treated HDFs, DMSO-treated HDFs or HUVECs in 100 μl PBS or the same volume PBS without cells were intramuscularly injected into the ischemic hindlimb of BALB/C nude mice, Laser Doppler perfusion images (C) and quantitative analysis of blood flow D showed improved limb perfusion, Data are presented as means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, n = 5. The representative pictures of every group (F), E and H staining of paraffin sections and the fluorescence intensity of FITC-BSL1 (E) at 28 day showed the change of hindlimb and the number of functional endothelial cells. The necrotic area (G) and the Capillary density (H) is quantified by H&E staining and FITC-BSL1 staining. Scale bar: 200 μm (H and E); 50 μm (FITC-BSL1). Data are presented as means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3