Fig. 8

HDFs treated with CPP secrete angiogenesis-related factors. A–C mRNA expression levels of VEGF, FGF-2 and PDGF-BB in HDFs treated with or without CPP (10 μM) for 10 days (D10) were analyzed by qPCR. (D)-(I) HDFs were treated with or without CPP for 10 days (D10), and protein levels of VEGF, FGF-2 and PDGF-BB were determined by Western Blot (D), (F) and (H). β-actin (ACTB) was used as a loading control. Quantitation of bands in the Western blot bands (D), (F) and (H) are shown in (E), (G) and (I). Data are presented as means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3