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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mesenchymal stem cell aggregation mediated by integrin α4/VCAM-1 after intrathecal transplantation in MCAO rats

Fig. 5

Expression of genes involved in intercellular adhesion in 2D versus 3D cultured MSCs. A, B Flow cytometry (A) and immunofluorescence (B) analyses of E-cadherin expression in 2D MSCs. C Skin epidermal cells (HaCaT) were used as a positive control for immunofluorescence analysis of E-cadherin. Scale bar, 50 μm (B, C). D Some differentially expressed genes involved in cell adhesion between 2D and 3D cultured MSCs were revealed by RNA-Seq analysis. E Flow cytometry analysis of 2D and 3D cultured MSCs for cell surface expression of integrin α4 and VCAM-1. Control cells were incubated with isotype IgG

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Stem Cell Research & Therapy

ISSN: 1757-6512