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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Cryopreservation does not change the performance and characteristics of allogenic mesenchymal stem cells highly over-expressing a cytoplasmic therapeutic transgene for cancer treatment

Fig. 2

Cryopreservation of MSC overexpressing CD::UPRT::GFP does not alter MSC markers and differentiation potential. (A) MSC positive markers CD44 and CD90, and negative marker CD34 were stained using PE-conjugated antibodies and assessed using flow cytometric analysis against an isotype control. Table indicates percentage of CD44 + , CD90 + and CD34 − cells within the respective samples. (B) Trilineage differentiation potential of cryopreserved MSC overexpressing CD::UPRT::GFP relative to unmodified and freshly modified samples. Oil Red O (Sigma), Alizarin Red S (Sigma) and Alcian Blue (Sigma), were used to stain for oil droplets, calcium deposits and proteoglycan in each of the differentiated samples, respectively. Experiment was performed in triplicates (n = 3), representative images are presented

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Stem Cell Research & Therapy

ISSN: 1757-6512