Fig. 4

A. PKH-26 labeling of hUC-MSC exosomes taken up by RAW264.7 cells. Scale bar = 20 μm. B. Oil red O staining of foamy RAW264.7 cells (left panel). (a) Without any treatment; (b) Ox-LDL treatment for 48 h; (c) Ox-LDL treatment for 48 h after control medium (CM) extract treatment for 24 h; d. Ox-LDL treatment for 48 h after UC-MSC treatment for 24 h. Three fields were randomly selected to calculate the number of red lipid droplets and the number of cells. The fold change is shown by the histogram (right panel). C. Quantitative PCR results showing the endogenous mRNA levels of TNF-α, IL-6 and IL-1β in RAW264.7 cells. CM represents control medium extract treatment, and Ex represents UC-MSC exosome treatment. The independent experiment has been repeated for three times. *p < 0.05; **p < 0.01. D. Detection of the M2 polarization markers CD206, Arginase-1 and IL-10 in Ox-LDL-treated RAW264.7 cells by quantitative PCR. CM represents control medium extract treatment, and Ex represents UC-MSC exosome treatment. GAPDH mRNA served as reference standard. *p < 0.05; **p < 0.01