Fig. 7

Qualitative evaluation of GLUT3 and GLUT5 protein expression during early chondrogenic commitment by immunostaining. A Representative pictures of n = 3 donors, here donor #2 is represented. BMSCs, cultured in monolayer and in the presence of chondrogenic medium, were stained against GLUT5 and GLUT3. The intensity of fluorescent secondary antibody is directly proportional to the production of GLUT5 and GLUT3, while DAPI (blue) stains cell nuclei. Scale bar (bottom right picture) = 100 µm (10×). B Mean intensity and area of GLUT3 and GLUT5 positive cells. Data are expressed as mean ± SD (*p ≤ 0.05, **p ≤ 0.01 vs HG group). N = 3 BMSC donors