Fig. 4

KW-2449 and VPA restores human MECP2-KO neurons in 2D culture. A Representative image of immunofluorescence staining for MAP2 in PX330 and MECP2-KO neurons treated with 1 µM KW-2449 or 10 µM VPA during days 20–40 of neural differentiation. B–E Quantification of total dendritic length (B), dendritic branch number (C), and intersection numbers (D, E) of MAP2⁺ neurons in A. n > 60 neurons from 3 independent experiments per group. *p < 0.05, **p < 0.01, ***p < 0.001. Data are shown as mean ± SEM; Two-tailed Student’s t-test