Table 1 Primers sequences used for qRT-PCR
Gene symbol | Primer sequence | Accession number | Annealing temperature °C | Amplicon size bp |
|---|---|---|---|---|
Bdnf | Forward primer: 5′-CGGTCACCGTCCTTGAAAA-3′ Reverse primer: 5′-GGATTGCACTTGGTCTCGTAGAA-3′ | NM_001009828.1 | 60 | 76 |
Gapdh | Forward primer: 5′-TGGAAAGCCCATCACCATCT-3′ Reverse primer: 5′-CAACATACTCAGCACCAGCATCA-3′ | NM_001009307.1 | 60 | 77 |
Sdf-1 | Forward primer: 5′ACAGATGTCCTTGCCGATTC-3′ Reverse primer: 5′-CCACTTCAATTTCGGGTCAA -3′ | XM_006937984.5 | 59 | 152 |
Ngf | Forward primer: 5′-GCAGGGCAGACCCGCAACAT-3′ Reverse primer: 5′-GCACCACCCGCCTCCAAGTC-3′ | XM_045033321.1 | 60 | 140 |