Fig. 8

ABCB5⁺ MSCs therapy improve microvascular density in ApoE^−/− mice after DLFA. A, C Representative pictures of IHC staining for A CD31 and B vWF (black arrow) in bilateral gastronemicus muscles (GM) of the ABCB5⁺ MSCs treated as well as control mice (scale bar: 200 μm). B, D Comparison of the ratio of B CD31 and D vWF positive areas in GM sections of ischemic and non-ischemic hindlimbs between control and ABCB5⁺ MSCs groups. E LDH ratios levels in VL tissues derived from controls and the ABCB5⁺ MSCs group; F Mb ratios level in VL tissues derived from both groups. Data were represented as mean ± SD, n = 10 in ABCB5⁺ MSCs group, n = 12 in controls group, ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001