Fig. 4

Effect of siH19-MSCs derived matrices on pre-adipocytes phenotype. a Osteogenic, adipogenic and chondrogenic markers expression levels assessed by RT-qPCR, 7 days after repopulation with pre-adipocytes on decellularized matrices (N = 6). b Representative image and quantification of the percentage of positive cells for the Oil Red O staining after 3 days of culture on decellularized matrices (Hematoxylin: blue-purple color; Oil Red O⁺ staining: red color) (N = 6; scale: 100 μm). c Quantification of LDH levels on the conditioned-media of pre-adipocytes cultured on decellularized matrices (N = 6). d Correlation of trabecular separation (Tb.Sp) with the percentage of adipose tissue in bone samples from patients with fragility fractures (N = 16) (left panel). Representative images (scale: 100 μm) and correlation coefficient of trabecular separation (Tb.Sp) and age (N = 17) (right panel).