Fig. 5
From: Human cortical spheroids with a high diversity of innately developing brain cell types
Generation of innately developing endothelial lineage cell populations in neuron-, astrocyte-, oligodendrocyte- and microglia-containing day-150 (d150) human cortical spheroids (hCSs). A Levels of mRNA expression of endothelial lineage transcripts SPARC, COL4A2, VWF and CD143 in H9 embryonic stem cells (ESCs) and d150 hCSs (batch-1). Independent samples T-test: *p < 0.05. B Levels of mRNA expression of SPARC and COL4A2 in H9 ESCs (day 0) and hCSs at various culturing time points. Significances are relative to d0. ANOVA with Dunnett’s T3 post hoc comparisons: ***p < 0.001, **p < 0.01, *p < 0.05, #p < 0.1. Error bars represent the standard error of the mean. Each data point in (A, B) represents the level of mRNA expression in one spheroid. C Representative confocal images of PECAM1-positive cells in d150 hCSs. D Ultrastructural transmission electron microscopy (TEM) images of endothelial-like cells (E) in d150 hCSs