Fig. 6
From: Human cortical spheroids with a high diversity of innately developing brain cell types
Comparative analysis of the cell-type composition of day-150 (d150) human cortical spheroids (hCSs), and the cell-type compositions of human fetal brain at various gestational weeks (GWs) and fetal brain regions. A Cell-type composition of d150 hCSs (batch-1) as quantified via immunocytochemistry (ICC) analysis of cell-type markers. B Percentages estimated per cell type in d150 hCSs (batch-1 and batch-12) based on the single-cell-type cluster-specific markers in GW8 to GW26 prefrontal cortex as deduced from the RNA-seq data of Zhong et al. [33]. C Percentages estimated per cell type in d150 hCS (batch-1 and batch-12) based on the single-cell-type cluster-specific markers in frontal cortex (FC), parietal cortex (PC), temporal cortex (TC), occipital cortex (OC) and the inferior surface (IS) of the midgestational (GW22/23) human fetal brain as deduced from the RNA-seq data of Fan et al. [32]. D Percentages of overlap between cell-type markers per brain region and transcripts expressed =  > 0.5 FPKM in (batch-1 and batch-12) d150 hCSs. The dotted lines in (B, C) represent the median of the summed squared distance to the quantifications based on ICC. NPC: neural progenitor cell; EXC: excitatory neuron; INH: inhibitory neuron; AST: astrocyte; OPC: oligodendrocyte precursor cell; pOL: pre-myelinating oligodendrocyte; MG: microglia; ENDO: endothelial cell