Fig. 7
From: Human cortical spheroids with a high diversity of innately developing brain cell types
Stimulation of day-150 (d150) human cortical spheroids (hCSs) with a cocktail of pro-inflammatory factors induces a neuroinflammatory response. A Levels of mRNA expression of the neuroinflammatory cytokines IL-6, CXCL8, CCL20 and CCL5, and the mitochondrial stress marker SOD2 in unstimulated hCSs (batch-12) and hCSs stimulated with a cocktail of LPS (100 ng/ml, 24 h) followed by TNFα and IL1β (5 ng/ml each, 24 h) (L + T/I). Independent samples T-test: ***p < 0.001. B Levels of mRNA expression of cell-type-specific markers in unstimulated and stimulated hCSs (batch-12). Independent samples T-test: **p < 0.01, *p < 0.05. Error bars represent the standard error of the mean. Each data point in (A, B) represents the level of mRNA expression in one spheroid