Fig. 1

Characterization of primary culture derived from the stromal vascular fraction of interscapular adipose tissue of newborn mice. A Analysis by flow cytometry for expression of the Sca1, CD29, CD44, CD90, CD11b, and CD45 markers. In each panel, the red line represents the marker of interest, the gray line the isotype control, and the upper part indicates the percentage of the negative and positive populations for each marker. In vitro adipogenic (B), chondrogenic (C), and osteogenic (D) differentiation was assessed by oil Bodipy staining of neutral lipids, safranin-O staining of cartilage, and alizarin red staining of hydroxyapatite, respectively. In each case, the result of the differentiation is shown in the upper image and undifferentiated control in the lower image. E Corresponds to a positive control of osteogenic differentiation using human MSC stained with alizarin red