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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Spheroids derived from the stromal vascular fraction of adipose tissue self-organize in complex adipose organoids and secrete leptin

Fig. 5

Three-dimensional structure and viability of AS differentiated with InRo protocol. SVF spheroids were subjected to adipogenic differentiation with the InRo protocol for 0, 5, and 15 days (DD0 to DD15, respectively). AS were stained for neutral lipids (Bodipy, green), dead cells (propidium iodide, PI, red) and nuclei (Hoechst, blue). Positive controls for PI staining were post-fixation AS permeabilized with Triton X-100 0.05%. 3D reconstruction and Z-projection based on Z-Stack is shown. Major divisions in 3D reconstruction grid and scale bar of 100 µm

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Stem Cell Research & Therapy

ISSN: 1757-6512