Fig. 6

AS differentiated with the InRo protocol have a complex inner structure. A 3D reconstruction of confocal Z-stack of AS at day 15 of adipogenic differentiation with InRo protocol and stained for nuclei (Hoechst, blue) and neutral lipid (Bodipy, green) detection. B–F Ultrastructural analysis of AS at day 15 of differentiation with InRo protocol. (C and D) Reconstruction of cross-sectioned AS with a montage of images obtained with a magnification of 390x (scale bar of 100 µm). E Adipocytes with single and large lipid droplets (LD) in the periphery of the spheroids. Elongated cells (arrow) are organized in 1 to 3 layers of lipid-laden cells. F The center of AS shows no evidence of necrosis and cells with small lipid droplets (nucleus N1) or no lipid build up (nucleus N2) are embedded in abundant loose cellular matrix (ECM). Images in (E) and (F) were captured at 790 × magnification, 20 µm scale bar