Fig. 3
From: Cell-type dependent regulation of pluripotency and chromatin remodeling genes by hydralazine
Expression of pluripotency genes by the effect of hydralazine (HYD) and valproic acid (VPA) in adult and neonatal fibroblasts. Adult and neonatal fibroblasts were treated for 72 h with 30 µM HYD, 1 mM VPA or the combination of both. Total RNA was extracted for each group, and RT-qPCR assays were performed for OCT4 (panel A), NANOG (panel B), c-Myc (panel C) and KLF4 (panel D) genes. Gene expression analysis were performed by technical triplicate of three biological replicates. Values are expressed as mean ± standard error of the median. The Mann–Whitney U test was used for comparisons between each group. *P < 0.05