Fig. 4
From: Cell-type dependent regulation of pluripotency and chromatin remodeling genes by hydralazine
CpG methylation analysis of OCT4 and NANOG promoter regions in adult and neonatal fibroblasts. Panel A, schematic representation of CpG methylation (5′-CCGG-3′) sites at OCT4 and NANOG promoters. CpG methylation analysis of OCT4 (panel B and D) and NANOG (panel C and E) promoters in adult and neonatal fibroblasts. Fibroblasts were treated for 72 h with 30 µM HYD. Gene expression analysis were performed by technical duplicate of three biological replicates. Values are expressed as mean ± standard error of the median. The Mann–Whitney U test was used for comparisons between groups