Fig. 5
From: Cell-type dependent regulation of pluripotency and chromatin remodeling genes by hydralazine
Evaluation of reprogramming efficiency by the effect of hydralazine (HYD) in adult and neonatal fibroblasts. Panel A, iPSC generation scheme with or without 30 µM HYD (w/wo HYD). hESC, human embryonic stem cells; iMEF, inactivated mouse embryonic fibroblasts. Panel B, representative images of the characteristic morphology of iPSC colonies (passage No. 3) from adult and neonatal fibroblasts. White bar in each micrograph corresponds to 400 µm. Colony number of iPSC with or without HYD treatment in adult (panel C) and neonatal (panel D) fibroblasts. Values are expressed as mean ± SEM from three independent experiments. Two-tailed Student's t test was used for comparisons between groups. Detection of pluripotency markers OCT4, NANOG, SOX2 and SEEA4 by immunofluorescence assays on iPSC colonies generated from adult (panel E) and neonatal (panel F) fibroblasts. Images were taken with a 10 × objective lens. White bar in each micrograph corresponds to 400 µm