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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human umbilical cord mesenchymal stem cells conditioned medium exerts anti-tumor effects on KGN cells in a cell density-dependent manner through activation of the Hippo pathway

Fig. 4

Effect of UCMSCs-CM on apoptosis of KGN cells. KGN cells were implanted at high (High) and low (Low) density. A Flow cytometric analysis of apoptosis in KGN cells treated with UCMSCs-CM for 48 h. B Representative TUNEL stain images of KGN cells subjected to UCMSCs-CM treatment. Scale bar, 200 μm. C The quantitative results of flow cytometry assay. D Expression of Bax and Bcl-2 measured by western blot. Full-length blots are presented in Additional file 2: Fig. S5. E–G The quantitative results showing the relative expression levels of Bax and Bcl-2 proteins and the ratio of Bax/Bcl-2. β-actin was used as loading control. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NS No statistical difference

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Stem Cell Research & Therapy

ISSN: 1757-6512