Fig. 6

UCMSCs-CM activated the Hippo signaling in KGN cells at high density. KGN cells were implanted at high (High) and low (Low) density. A Expression of p-LAST1 (S909), LAST1, p-YAP (ser127), and YAP detected by western blot analysis. Full-length blots are presented in Additional file 2: Fig. S6. B KGNs pretreated with XMU-MP-1 and cultured with UCMSCs-CM treatment for 48 h. Expression of p-LAST1 (S909), LAST1, p-YAP (ser127), and YAP detected by western blot analysis. Full-length blots are presented in Additional file 2: Fig. S7 C–D The quantitative results showing the ratio of p-LAST1/ LAST1 and p-YAP (ser127)/YAP. E Representative immunofluorescence images of KGN cells stained with anti-YAP (green), DAPI (blue). Scale bar, 50 μm. β -actin was used as loading control.*P < 0.05, **P < 0.01, ****P < 0.0001. NS No statistical difference