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Fig. 11 | Stem Cell Research & Therapy

Fig. 11

From: Highly-purified rapidly expanding clones, RECs, are superior for functional-mitochondrial transfer

Fig. 11

Effect of cell passage frequency on mitochondrial quality in REC. A Representative images show the growth state of REC seeded with the same number of cells at different passages after 48 h and the cell viability of REC cells under different passages. B Representative fluorescence images showing the mitochondrial membrane potential of REC cells at different passages and the fluorescence intensity ratio of mitochondrial membrane potential levels (JC-1) in different passages. C Oxygen consumption rate of REC cells under different passages. D mtDNA content of REC cells under different passages. E The mean fluorescence intensity (Mito-Green) of REC cells under different passages. F RT-qPCR quantitatively analyzes the effect of ρ0 cells (receiving REC-donated mitochondria) on mtDNA content in the 2 and 1 Revisiting the environmental Kuznets curve in China: A spatial dynamic panel data approach 2 passages. G The fluorescence intensity ratio of mitochondrial membrane potential levels (JC-1) in the 2 and 12 passages ρ0 cells (receiving REC-donated mitochondria). Data were based on three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ns: no statistical significance between the indicated groups

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