Fig. 2
From: Highly-purified rapidly expanding clones, RECs, are superior for functional-mitochondrial transfer
Mitochondrial transfer from MSCs/RECs to ρ0 cells. A Representative image of co-culture showing ρ0 A549 cells (red) with MSC (green). B Representative image of co-culture showing ρ0 A549 cells (red) with the REC (green). C Representative image of co-culture showing ρ0 HeLa cells (red) with the MSC (green). D Representative image of co-culture showing ρ0 HeLa cells (red) with the REC (green). Arrowhead: green fluorescence of mitochondria from MSC/REC transmitted to the ρ0 cells containing red fluorescence, indicating successful transference. E Representative distribution of DiD-labeled ρ0 cells (red) and MitoTracker-labeled MSC/REC (green) in co-cultured cells analyzed by FCM. F The time course of the mitochondrial transfer ratio between REC/MSC and ρ0 A549 cells distributed at the Q2 phase and the percentage of this population over the total ρ0 A549 cells are presented. G The time course of the mitochondrial transfer ratio between REC/MSC and ρ0 HeLa cells distributed at the Q2 phase and the percentage of this population over the total ρ0 HeLa cells are presented. H Differences in the rate of mitochondrial transfer by REC to different ρ0 cell lines between 0 and 48 h are shown. Data were based on three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001