Fig. 5
From: Highly-purified rapidly expanding clones, RECs, are superior for functional-mitochondrial transfer
Mitochondrial transfer rates of REC and MSC to ρ0 cells under various inhibitors. MSC and REC donate the mitochondria to ρ0 A549 cells (A) and ρ0 HeLa cells (B) via multiple mechanisms. MSC and REC were co-cultured with ρ0 cells and treated with either Dynasore to inhibit endocytosis, Cytochalasin D (Cyto-D) to inhibitor TNT formation, Carbenoxolone (CBX) to inhibit all gap junction formation, and 0.4 µm Boyden chamber (0.4 µm BC) inhibits direct cell-to-cell contact. C REC donate the mitochondria to ρ0 A549 cells and ρ0 HeLa cells via multiple mechanisms. A horizontal line represents uninhibited control. The proliferation of ρ0 A549 cells (D) and ρ0 HeLa cells (E) when co-cultured with MSC or REC. carboxyfluorescein-succinimidyl ester (CFSE) labels ρ0 cells. Data were based on three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. ns: no statistical significance between the indicated groups