Fig. 7
From: Highly-purified rapidly expanding clones, RECs, are superior for functional-mitochondrial transfer
MtDNA recovery of ρ0 cells in the non-contact co-culture system. ρ0 cells acquired MSC and REC-derived mtDNA. A Total DNA from ρ0 cells, ρ0 + MSC cells, ρ0 + REC cells, REC, and MSC were isolated and analyzed by PCR for the presence of NADH dehydrogenase 1 (ND1), Cytochrome c oxidase I (COX1), and hypervariant region 2 (HVR2) in mtDNA and chromosome 1 segment for nuclear DNA (nDNA). Full-length gels are presented in Additional file 2. Note that the ρ0 cells exhibited no detectable mtDNA. Isolated RNA from ρ0 cells, ρ0 + MSC cells, ρ0 + REC cells, REC, and MSC were quantitatively analyzed using RT-qPCR for ND1 (B), COX1 (C), HVR2 (D) mtDNA content, and CH1 (E) nuclear DNA content. F RT-qPCR quantitatively analyzes the effect of REC on mtDNA and nDNA content in different ρ0 cell lines. All co-culture groups were in the non-contact co-culture system. Data were based on three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001