Table 1 Primer pairs to confirm establishment of ρ0 A549 and ρ0 HeLa cell lines
From: Highly-purified rapidly expanding clones, RECs, are superior for functional-mitochondrial transfer
Gene name | Primer pairs | Product size (bp) |
|---|---|---|
ND1 (mtDNA) | Forward 5′-ACC CCC GAT TCC GCT ACG ACC AAC-3′ | 164 |
Reverse 5′-GGT TTG AGG GGG AAT GCT GGA GAT-3′ | ||
COX1 (mtDNA) | Forward 5′-ACA CGA GCA TAT TTC ACC TCC G-3′′ | 336 |
Reverse 5′-GGA TTT TGG CGT AGG TTT GGT C-3′ | ||
HVR2 (mtDNA) | Forward 5′-CTC ACG GGA GCT CTC CAT GC-3’ | 401 |
Reverse 5′-CTG TTA AAA GTG CAT ACC GCC A-3′ | ||
CH1 (nDNA) | Forward 5′-GGC TCT GTG AGG GAT ATA AAG ACA-3′ | 98 |
Reverse 5′-CAA ACC ACC CGA GCA ACT AAT CT-3′ | ||
ACTB | Forward 5′-TGG CAC CCA GCA CAA TGA A-3′ | 186 |
Reverse 5′-CTA AGT CAT AGT CCG CCT AGA AGC A-3′ |