Fig. 6

Effect of Numb expression on the differentiation of WB-F344 cells. a Experimental flow chart. b Cell morphology and GFP expression after lentivirus transfection for 72 h (× 100). c, d Numb protein and mRNA levels (Full-length blots are presented in Additional file 2: Figs. 15 and 16). e, f CK19 immunofluorescence staining (× 600). g, h Immunofluorescence staining for Numb, RBP-Jκ and Hes1 (× 600). i, j The mRNA levels of CK19, Numb, RBP-Jκ and Hes1. *P < 0.05; **P < 0.01