Fig. 6

Tracking of injected clonal mesenchymal stem cells (cMSCs) and extracellular vesicles (EVs) in the treated uterine horns. A STEM121 immunofluorescence staining was used to detect transplanted clonal mesenchymal stem cells (cMSCs) in the endometrial tissue (I–IV); IgG control (V–VI), negative control (VII–VIII). Scale bar: 100 µm. B Labeling and ex vivo tracking of extracellular vesicles (EVs) by Calcein AM dye after in situ administration in the uterus at different time points: (I) Intrauterine injection of unlabeled EVs as a negative control. (II) Intrauterine injection of Calcein without EVs as staining control. Intrauterine injection of Calcein-labelled EVs at Day 0 (III), 7 day (IV), and 14 days (V) after EV treatment. The presence of Calcein in the uterine horns was visualized with UVI gel documentation