Skip to main content

Table 2 Main findings of the studies comparing miRNAs expression profile before and after the differentiation (studies of group II)

From: MicroRNAs-mediated regulation of the differentiation of dental pulp-derived mesenchymal stem cells: a systematic review and bioinformatic analysis

Author [reference]

Cell type

Cell source

Type of differentiation

Technique of differentiation assessment

Differentially expressed miRNAs

Technique of miRNA assessment

Liu et al. [13]

hDPSC

Third molar

Odontogenic/osteogenic

ARS, ALP staining

113 (63 ↑, 50 ↓)

Sequencing, qRT-PCR

Chen et al.* [15]

hDPSC

Premolar

Odontogenic/osteogenic

ARS, ALP staining

114 (24 ↑, 90 ↓)

Microarray, qRT-PCR

Hu et al.* [16]

hDPSC

Third molar

Odontogenic/osteogenic

ARS

28 (7 ↑, 21 ↓)

Sequencing, qRT-PCR

Song et al.* [17]

hDPSC

Third molar

Odontogenic/osteogenic

ARS

36 (22 ↑, 14 ↓)

Microarray, qRT-PCR

Dernowsek et al.* [49]

SHED

Human deciduous tooth

Odontogenic/osteogenic

ARS, ALP activity

21 (11 ↑, 10 ↓)

Microarray, qRT-PCR

Gay et al.* [55]

hDPSC

Third molar

Odontogenic/osteogenic

ARS

6 (2 ↑, 4 ↓)

Microarray, qRT-PCR

Hara et al.* [64]

hDPSC

Third molar and premolar

Odontogenic/osteogenic

ARS, ALP staining

75 (60 ↑, 15 ↓)

Locked nucleic acid-based Microarray, qRT-PCR

Gong et al. [14]

hDPSC

Third molar and premolar

Odontogenic/osteogenic

ARS, VKS

22 (12 ↑, 10 ↓)

Microarray, qRT-PCR

  1. ↓Down-regulated during differentiation
  2. ↑Up-regulated during differentiation
  3. Studies marked with * are in common with Table 1. They selected specific miRNA(s) among differentially expressed miRNAs and, as a complementary step, investigated their regulatory effect on the differentiation of hDP-MSCs
  4. ALP, Alkaline phosphatase; ARS, Alizarin red staining; qRT-PCR, Quantitative reverse transcription polymerase chain reaction; VKS, von Kossa staining