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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Low-intensity pulsed ultrasound promotes mesenchymal stem cell transplantation-based articular cartilage regeneration via inhibiting the TNF signaling pathway

Fig. 2

Effects of LIPUS on the chondrogenic differentiation of hUC-MSCs. The hUC-MSCs were stimulated by 20 min/day LIPUS stimulation for 7 days before analyses. a Representative Alcian blue staining images showing the differentiation level of hUC-MSCs into chondrocytes; scale bar: 200 μm. b Quantification data of the proteoglycan production for the Alcian blue staining results. c–e Representative immunofluorescence images showing the expression of cytoskeleton actin (c), and two marker genes of chondrogenic differentiation, SOX-9 (d) and ACAN (e); scale bar: 100 μm. f qPCR analysis of the expression of SOX-9, COL-II, COL-X, ACAN, and COMP. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512