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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Low-intensity pulsed ultrasound promotes mesenchymal stem cell transplantation-based articular cartilage regeneration via inhibiting the TNF signaling pathway

Fig. 3

Effects of LIPUS stimulation on the proliferation and inflammation of C28/I2 cells. a Flow cytometry analysis for cell apoptosis of C28/I2 cells stimulated with LIPUS. b Quantification data for apoptosis rate of C28/I2 cells. c Flow cytometry analysis for cell cycle distribution of C28/I2 cells. d Quantification data for the cell cycle of C28/I2 cells. e Representative live-dead staining images of C28/I2 stimulated with LIPUS; scale bar: 200 μm. f qPCR analysis of the expression of TNF-α, IL-1β, and IL-6 in C28/I2 cells, after LPS treatment (to produce inflammation) and LIPUS stimulation. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3)

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