Fig. 4

Transcriptome sequencing analysis. a Cluster heat map of mRNA expression of Ctrl, US-70, and US-100 samples. b A volcano plot comparing the DEGs between the US-70 and Ctrl groups (Down-regulated genes showed in green and up-regulated genes showed in red; cutoff: p-value < 0.05 and |log₂ FC|> 1). c–e KEGG pathway term analysis (c), KEGG pathway analysis (d), and GO pathway analysis of the DEGs (e) between the US-70 and Ctrl groups. f PPI network analysis of the core regulatory genes of the DEGs between the US-70 and Ctrl groups