Skip to main content
Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Low-intensity pulsed ultrasound promotes mesenchymal stem cell transplantation-based articular cartilage regeneration via inhibiting the TNF signaling pathway

Fig. 5

Effects of TNF signaling pathway in LIPUS stimulation-induced hUC-MSC chondrogenic differentiation. a qPCR analysis of the expression of inflammatory factor genes TNF-α, IL-1β, and CXCL8. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3). b Representative immunofluorescence images showing the expression level of inflammatory factor TNF-α; scale bars: 50 μm and 100 μm, respectively. c Quantification data of the proteoglycan production for the Alcian blue staining results. d Representative Alcian blue staining images showing the differentiation level of hUC-MSCs into chondrocytes; scale bar: 200 μm. e–f Representative immunofluorescence images showing the expression of the chondrogenic differentiation markers SOX-9 (e) and COL-II (f); scale bar: 100 μm

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512