Fig. 2

a Immunofluorescence analysis showed approximately similar biomarker expression of hLMSCs in SM for ocular surface marker (Pax6⁺), stem-cell biomarkers (ABCG2⁺, P63α⁺, ABCB5⁺) and the mesenchymal biomarkers (VIM⁺, CD90⁺, CD105⁺, CD 34⁻, HLA-DR⁻ and CD45⁻) with respect to the cells in control medium; b the expression of MSC markers in hLMSCs grown in both the media was quantified using flow cytometry. More than 97% of cells were positive for CD105, CD90, and CD73, whereas less than 1% showed expression for negative markers CD45 and HLA-DR and approx. 6% of total cells were positive for CD34; c graphical representation of flow cytometry data. Blue: DAPI; Scale: 50 µM; Magnification: × 20 (all other micrographs) and 20 µM (CD73 of DMEM/F12 with 2% FBS; × 40 magnification)