Expansion and characterization of human limbus-derived stromal/mesenchymal stem cells in xeno-free medium for therapeutic applications

Sahoo, Abhishek; Damala, Mukesh; Jaffet, Jilu; Prasad, Deeksha; Basu, Sayan; Singh, Vivek

doi:10.1186/s13287-023-03299-3

Stem Cell Research & Therapy

Table 1 List of primers used in this study for gene expression analysis

From: Expansion and characterization of human limbus-derived stromal/mesenchymal stem cells in xeno-free medium for therapeutic applications

Sl. no	Genes	Primer sequence		Size (bp)
1	LUMICAN	Fwd	GCACAATCGGCTGAAAGAGG	228
1	LUMICAN	Rev	TCAGCCAGTTCGTTGTGAGA	228
2	IL10	Fwd	GCTGGAGGACTTTAAGGGTTACCT	109
2	IL10	Rev	CTTGATGTCTGGGTCTTGGTTCT	109
3	IL 6	Fwd	GCGATGGAGTCAGAGGAAACT	218
3	IL 6	Rev	AGTGACTCAGCACTTTGGCA	218
4	COL1A1	Fwd	GTCACCCACCGACCAAGAAACC	121
4	COL1A1	Rev	AAGTCCAGGCTGTCCAGGGATG	121
5	COL5A1	Fwd	TTCAAGCGTGGGAAACTGCT	115
5	COL5A1	Rev	GGTAGGTGACGTTCTGGTGG	115
6	TGFβ1	Fwd	TACCTGAACCCGTGTTGCTCTC	122
6	TGFβ1	Rev	GTTGCTGAGGTATCGCCAGGAA	122
7	COL3A1	Fwd	TGAAAGGACACAGAGGCTTCG	532
7	COL3A1	Rev	GCACCATTCTTACCAGGCTC	532
8	P63α	Fwd	ACCTGGAAAACAATGCCCAGA	369
8	P63α	Rev	GAGGTGGGGTCATCACCTTG	369
9	VIM	Fwd	GGACCAGCTAACCAACGACA	178
9	VIM	Rev	AAGGTCAAGACGTGCCAGAG	178
10	CD105	Fwd	CGGTGGTCAATATCCTGTCGAG	109
10	CD105	Rev	AGGAAGTGTGGGCTGAGGTAGA	109
11	CD90	Fwd	AGCATCGCTCTCCTGCTAAC	230
11	CD90	Rev	CTGGTGAAGTTGGTTCGGGA	230
12	CD73	Fwd	GGCTGCTGTATTGCCCTTTG	175
12	CD73	Rev	TACTCTGTCTCCAGGTTTTCGG	175
13	RUNX2	Fwd	CCACTGAACCAAAAAGAAATCCC	129
13	RUNX2	Rev	GAAAACAACACATAGCCAAACGC	129
14	CD45	Fwd	CTTCAGTGGTCCCATTGTGGTG	107
14	CD45	Rev	CCACTTTGTTCTCGGCTTCCAG	107
15	KERA	Fwd	GACACAGGACTCAACGGTGT	205
15	KERA	Rev	GTAGGAAAACTGGGTGGGCA	205
16	ALDH3A1	Fwd	CAGTTACCGGGAGAGGCTGT	345
16	ALDH3A1	Rev	GTGGCTCCGAGTGGATGTAG	345
17	SEMA3A	Fwd	AGACTCACTTGTACGCCTGTG	242
17	SEMA3A	Rev	CCCAAGAGTTCGGAAGATAGCAA	242
18	DCN	Fwd	ATGAAGGCCACTATCATCCTCC	135
18	DCN	Rev	GTCGCGGTCATCAGGAACTT	135
19	COL4A1	Fwd	TGTTGACGGCTTACCTGGAGAC	120
19	COL4A1	Rev	GGTAGACCAACTCCAGGCTCTC	120
20	PAX6	Fwd	ATAACCTGCCTATGCAACCC	208
20	PAX6	Rev	GGAACTTGAACTGGAACTGAC	208
21	IL1β	Fwd	CCTGTCCTGCGTGTTGAAAGA	149
21	IL1β	Rev	GGGAACTGGGCAGACTCAAA	149
22	TNFα	Fwd	CCCCAGGGACCTCTCTCTAATC	94
22	TNFα	Rev	GGTTTGCTACAACATGGGCTACA	94

Back to article page

ISSN: 1757-6512

Contact us

Submission enquiries: Access here and click Contact Us
General enquiries: info@biomedcentral.com