Fig. 3

Analysis of cryopreserved bone marrow-mesenchymal stem cells. Three independent samples per group were cryopreserved in liquid nitrogen for 3 months with two different formulations based on inactivated human platelet lysate and compared with a standard solution. A Cell viability and B recovery post-thaw, C recovery at 24-h culture post-thaw, D flow cytometry analysis before and after cryopreservation. E Immunomodulatory capacity of cryopreserved BM-MSCs measured as the percentage of non-proliferative T-cells relative to the activated peripheral blood mononuclear cell (PBMC) population. F Representative carboxyfuorescein succinimidyl ester dye (CFSE) histograms of activated PBMCs both in co-culture with fresh BM-MSCs or with thawed BM-MSCs that were cryopreserved in each solution. G Cell stability analysis measured at different time points. Data are shown as mean ± SEM. One-way ANOVA: differences between standard and ihPL-based solutions *P < 0.05; **P < 0.01; ***P < 0.001; differences between CeA and CeB ^#P < 0.05