Fig. 5
Analysis of cryopreserved neural stem cells. Two or three independent samples per group were cryopreserved in liquid nitrogen for 3 months with the inactivated human platelet lysate cryopreservation solution CeA, and compared to the standard solution (FBS + 10%Me2SO) and to the commercial products CryoStor® CS10 and STEM-CELLBANKER® DMSO FREE-GMP Grade (SCB). A Cell viability and B recovery post-thaw. C Representative phase-contrast microphotographs of cells one passage after thawing (scale bar: 100 µm). D Cell proliferation (population doublings). Representative histograms of the quantification of surface marker expression: E CD133, F PODXL, G ILRAP and H MHCII in thawed cells. The results are presented as mean ± SEM. One-way ANOVA: differences between fresh and cryopreserved cell values/between standard and cryopreservation solutions under assessment: *P < 0.05; ***P < 0.001; ****P < 0.0001