Fig. 6

Quantification of components and stability analysis of inactivated human platelet lysate-based solutions for cell cryopreservation. Cryopreservation properties of stored solutions were assessed after long-term storage of 12 and 18 months at − 20 °C. Quantification of components: A Dot graph representation of total protein, B albumin and C immunoglobulin G (IgGs) quantification and D pH measurement. Data are shown for one batch per solution. Stored solutions were tested to check whether they maintained their cryopreservation properties. Fibroblasts were cryopreserved and analyzed immediately after thawing: E cell viability and F recovery measurement. G Bar graph representation of cell recovery after 24-h cell culture. H Cell proliferation (population doubling). I representative phase-contrast microphotographs of cells one passage after thawing (scale bar: 50 µm). Data are shown as mean ± SEM