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Table 1 Description of conditions and reagents used for bioengineered tissue cryopreservation

From: Bioengineered tissue and cell therapy products are efficiently cryopreserved with pathogen-inactivated human platelet lysate-based solutions

Cryoprotective solutions

 

Control 1

70% DMEM + 20% FBS + 10% DMSO

Control 2

Cryostor® CS10

hPL-based

 

  10% DMSO

Solution Ti1

  10% DMSO

Solution Ti2

  5% DMSO

Solution Ti3

ihPL-based

 

  10% DMSO

Solution Ti4

  5% DMSO

Solution Ti5

Freezing method

Freezing EVA cryobagsa

Time in liquid N2 (days)

7

GMP laboratory

UPRC Seville

  1. DMSO dimethyl sulfoxide, hPL human platelet lysate, ihPL inactivated human platelet lysate, DMEM Dulbecco's Modified Eagle’s Medium, FBS fetal bovine serum, EVA ethylene–vinyl acetate, UPRC Unidad de Producción y Reprogramación Celular
  2. aSee Additional file 1
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Stem Cell Research & Therapy

ISSN: 1757-6512