Table 2 Description of conditions and reagents used for cell cryopreservation

Cryoprotective solution

 Control 1 (standard)

4.5% HSA + 10% DMSO

CryoStor® CS10

90%FBS + 10% DMSO

 Control 2

Not applicable

SCB

CryoStor® CS10

 Control 3

Not applicable

Not applicable

SCB

 ihPL-based 5% DMSO

Solution CeA

Solution CeA

Solution CeA

Solution CeB

Solution CeB

Not applicable

No. of cells

10 × 10⁶

1 × 10⁶ //  10 × 10⁶

0.5 × 10⁶

Freezing method

Nunc™ cryovials

Nunc™ cryovials

Nunc™ cryovials

Controlled-rate freezer^a

Mr. Frosty

Mr. Frosty

Time in liquid N₂ (months)

3

9

3

Thawing method

Water bath (37 °C)

Water bath (37 °C)

Water bath (37 °C)

GMP laboratory

HURS Cordoba

UPRC Seville

UPRC Seville